Home Publications Software Request for materials People Alumni Microscopy Teaching Contact
 

Lab Members

 

For full contact information click here

 

Group Leader

M. Cristina Cardoso graduated in biology at the University of Lisbon, Portugal (1986). After finishing her doctoral thesis on phage molecular genetics (1990), she went on to her post-doctoral training in the group of Dr. B. Nadal-Ginard at the Harvard Medical School (Boston, USA) where she worked on inducing retro-differentiation in terminally differentiated cells. During this time, she started to explore the interplay between the mammalian cell nucleus landscape and (epi)genome metabolism. In 1995, she became a group leader at the Franz Volhard Clinic (Charité-Berlin, Germany) and from 1997 to 2008 at the Max Delbrück Center for Molecular Medicine (Berlin). Since 2008, she has established her group at the Technische Universität Darmstadt.

Link to publications page

   

Secretary

Katrin Schallert is responsible to manage the secretariat of the work group. She is in charge for accounting and secretary matters. Accounting includes in particular the facts and figures of the research projects and in administrative matters. In the secretary, she is responsible among other things, to purchase lab and office material, doing the bills and taking care about personnel matters.

 

 

   
   
Dianca Imblan

Diana Imblan graduated in environment protection at the School of Applied Sciences in Bingen. She is working as a technical assistant in the Cardoso lab and takes care of several databases (Library of papers, Inventory lists, Peptides) and is supporting Manuela in protein purification.

   

Anne Lehmkuhl takes the best care of our cell cultures and is therefore absolutely not replaceable. She is all ears for special wishes and keeps the lab running with all the time freshly prepared solutions and sterilized lab materials.

Ludwig, A. K.#, Zhang, P.#, Hastert, F. D., Meyer, S., Rausch, C., Herce, H. D., Muller, U., Lehmkuhl, A., Hellmann, I., Trummer, C., Storm, C., Leonhardt, H. and Cardoso, M. C. (2017). Binding of MBD proteins to DNA blocks Tet1 function thereby modulating transcriptional noise. Nucleic Acids Res 45: 2438-2457.

Zhang, P.#, Ludwig, A. K.#, Hastert, F. D., Rausch, C., Lehmkuhl, A., Hellmann, I., Smets, M., Leonhardt, H. and Cardoso, M. C. (2017). L1 retrotransposition is activated by Ten-eleven-translocation protein 1 and repressed by methyl-CpG binding proteins. Nucleus 8: 548-562.

Becker, A., Allmann, L., Hofstatter, M., Casa, V., Weber, P., Lehmkuhl, A., Herce, H. D. and Cardoso, M. C. (2013). Direct homo- and hetero-interactions of MeCP2 and MBD2. PLoS One 8: e53730.

Bertulat, B., De Bonis, M. L., Della Ragione, F., Lehmkuhl, A., Milden, M., Storm, C., Jost, K. L., Scala, S., Hendrich, B., D'esposito, M. and Cardoso, M. C. (2012). MeCP2 dependent heterochromatin reorganization during neural differentiation of a novel Mecp2-deficient embryonic stem cell reporter line. PLoS One 7: e47848.

 

   
Manu

Manuela Milden studied Biotechnology at the University of Applied Sciences Darmstadt, Germany. Her diploma thesis was finished in 2008 in the department of molecular biology. Now she takes care of the biochemistry lab, the antibodies and our Äkta system.

Bertulat, B., De Bonis, M. L., Della Ragione, F., Lehmkuhl, A., Milden, M., Storm, C., Jost, K. L., Scala, S., Hendrich, B., D'esposito, M. and Cardoso, M. C. (2012). MeCP2 dependent heterochromatin reorganization during neural differentiation of a novel Mecp2-deficient embryonic stem cell reporter line. PLoS One 7: e47848.

Jost, K. L., Rottach, A., Milden, M., Bertulat, B., Becker, A., Wolf, P., Sandoval, J., Petazzi, P., Huertas, D., Esteller, M., Kremmer, E., Leonhardt, H. and Cardoso, M. C. (2011). Generation and Characterization of Rat and Mouse Monoclonal Antibodies Specific for MeCP2 and Their Use in X-Inactivation Studies. PLoS One 6: e26499.

 

 
   
 

 

Hadassa Campos-Heiser graduated in Biomedicine at the Centro Universitário de Maringá (UniCesumar) (2012) and hold a Master’s degree in Medical Science from the Medical School of the University of Sao Paulo (2015). After the Master she worked as Research Assistant in Population Genetics and Bioinformatics at the Laboratory of Genetics and Molecular Cardiology (LGCM-InCor). In 2017 she joined the Cardoso Lab where she is working on the identification and epigenetic characterization of the Origins of DNA Replication. Besides her scientific work she also enjoys photography, dancing and reading topics such as history, economics, physics and stars. She is a nature lover.

da Silva-Malta, M. C. F., Sales, C. C., Guimarães, J. C., de Cássia Gonçalves, P., Chaves, D. G., Santos, H. C., da Costa Pereira, A., Ribas, J. G., de Freitas Carneiro-Proietti, A. B., Martins, M.L. (2017). The Duffy null genotype is associated with a lower level of CCL2, leukocytes and neutrophil count but not with the clinical outcome of HTLV-1 infection. J Med Microbiol.

Martins, M. L., da Silva, A. R., Santos, H. C., Alves, M. T., Schmidt, L. C., Vertchenko, S. B., Dusse, L. M. S., Silva Malta, M. C. F. D. (2017). Duffy blood group system: New genotyping method and distribution in a Brazilian extra-Amazonian population. Mol Cell Probes 35: 20-26.

Egan, K. J., Campos Santos, H., Beijamini, F., Duarte, N. E., Horimoto, A. R., Taporoski, T. P., Vallada, H., Negrão, A. B., Krieger, J. E., Pedrazzoli, M., Knutson, K. L., Pereira, A. C., von Schantz, M. (2017). Amerindian (but not African or European) ancestry is significantly associated with diurnal preference within an admixed Brazilian population. Chronobiol Int 34(2): 269-272.

Tofoli, F. A., Dasso, M., Morato-Marques, M., Nunes, K., Pereira ,L. A., da Silva, G. S., Fonseca, S. A., Costas, R. M., Santos, H. C., da Costa Pereira, A., Lotufo, P. A., Bensenor, I. M., Meyer, D., Pereira, L. V. (2016). Increasing The Genetic Admixture of Available Lines of Human Pluripotent Stem Cells Sci Rep. 6: 34699.

Bernardez-Pereira, S., Gioli-Pereira, L., Marcondes-Braga, F. G., Santos, P. C., Spina, J. M., Horimoto, A. R., Santos, H. C., Bacal, F., Fernandes, F., Mansur, A. J., Pietrobon, R., Krieger, J. E., Mesquita, E. T., Pereira, A. C. (2016). Genomic ancestry as a predictor of haemodynamic profile in heart failure. Open Heart 3(2): e000434.

Dinardo, C. L., Santos, H. C., Vaquero, A. R., Martelini, A. R., Dallan, L. A., Alencar, A. M., Krieger, J. E., Pereira, A. C. (2015). Smoking and Female Sex: Independent Predictors of Human Vascular Smooth Muscle Cells Stiffening. PLoS One 10(12): e0145062.

Scalco, R. C., Gonçalves, F. T., Santos, H. C., Cardena, M. M. S. G., Tonelli, C. A., Funari, M. F. A., Aracava, R. M., Pereira, A. C., Fridman, C., Jorge, A. A. L. (2017). Growth hormone insensitivity with immune dysfunction caused by a STAT5B mutation in the south of Brazil: evidence for a founder effect. Genet Mol Biol 40(2): 436-441.

Egan, K. J., von Schantz, M., Negrão, A. B., Santos, H. C., Horimoto, A. R., Duarte, N. E., Gonçalves, G. C., Soler, J. M., de Andrade, M., Lorenzi-Filho, G., Vallada, H., Taporoski, T. P., Pedrazzoli, M., Azambuja, A. P., de Oliveira, C. M., Alvim, R. O., Krieger, J. E., Pereira, A. C. (2016). Cohort profile: the Baependi Heart Study-a family-based, highly admixed cohort study in a rural Brazilian town. BMJ Open 6(10): e011598.

Santos, H. C., Horimoto, A. V. R., Tarazona-Santos, E., Rodrigues-Soares, F., Barreto, M. L., Horta, B. L., Lima-Costa, M. F., Gouveia, M. H., Machado, M., Silva, T. M., Sanches, J. M., Esteban, N., Magalhaes, W. C. S., Rodrigues, M. R., Kehdy, F. S. G., Pereira, A. C. (2016). A minimum set of ancestry informative markers for determining admixture proportions in a mixed American population: the Brazilian set. European Journal of Human Genetics 24: 725–731.

Kehdy, F. S. G., Gouveia, M. H., Machado, M. M., Wagner, C. S., Horimoto, A. R., Horta, B. L., Moreira, R. G., Leal, T. P., Scliar, M. O., Soares-Souza, G. B., Rodrigues-Soares, F., Araújo, G. S., Zamudio, R., Sant Anna, H. P., Santos, H. C., Duarte, N. E., Fiaccone, R. L., Figueiredo, C. A., Silva, T. M., Costa, G. N., Beleza, S., Berg, Douglas E. Cabrera, L., Debortoli, G., Duarte, D., et al. (2015). Origin and dynamics of admixture in Brazilians and its effect on the pattern of deleterious mutations. Proceedings of the National Academy of Sciences of the United States of America 28: 201504447.

Lima-Costa, M. F., Rodrigues, L. C., Barreto, M. L., Gouveia, M., Horta, B. L., Mambrini J., Kehdy, F. S. G., Pereira, A., Rodrigues-Soares, F., Victora, C. G., Tarazona-Santos, E. & Epigen-Brazil group. (2015). Genomic ancestry and ethnoracial self-classification based on 5,871 community-dwelling Brazilians (The Epigen Initiative). Scientific Report (Nature) 5: 9812.

Negrão, A. B., Pereira, A. C., Guindalini, C., Santos,H. C. Messas, G. P. Laranjeira, R., Vallada, H. (2013). Butyrylcholinesterase Genetic Variants: Association with Cocaine Dependence and Related Phenotypes. Plos One 8: 80505.

Santos, H. C., Fragoso, T. M., Machado-Coelho, G. L., Do Nascimento, R. M., Mill, J. G., Krieger, J. E., Pereira, A. C. (2013). Self-declared ethnicity associated with risk factors of cardiovascular diseases in an urban sample of the Brazilian population: The role of educational status in the association. International Journal of Cardiology 1: IJCA-16230.

 

   

Emilia Rita De Caro is currently contributing to an interdisciplinary project of the departments of biology and material sciences and engineering. Aim of the collaboration is to establish a surface biology-testing unit (SuBiTU) that allows standardized in vitro testing of bone-implant surfaces. As a tool for implant characteriszation, SuBiTU will help to reduce animal testing and enable us to monitor early implant-surface-cell interactions. To this end we developed an implant suitable live cell chamber, established a stable cell lines, and currently optimizing 3D cell culture techniques for live cell microscopy.

 
Florian Hastert

Florian Hastert got both, his bachelor's and master's degree, from the TU Darmstadt. His bachelor's thesis was completed at the "GSI Helmholtz Centre for Heavy Ion Research" on the recruitment of proteins involved in chromatin remodeling following radiation induced DNA damage. In his master's thesis in the Cardoso lab he investigated the interplay of histone and DNA modifications. This is now also keeping him busy during his PhD.

plays drums with the BiophoniX

Zhang, P., Hastert, F. D., Ludwig, A. K., Breitwieser, K., Hofstätter, M. and Cardoso, M. C. (2017). DNA base flipping analytical pipeline, Biology Methods and Protocols 2: 1-12

Ludwig, A. K., Zhang, P., Hastert, F. D., Meyer, S., Rausch, C., Herce, H. D., Muller, U., Lehmkuhl, A., Hellmann, I., Trummer, C., Storm, C., Leonhardt, H. and Cardoso, M. C. (2017). Binding of MBD proteins to DNA blocks Tet1 function thereby modulating transcriptional noise. Nucleic Acids Res 45: 2438-2457.

Zhang, P., Ludwig, A.K., Hastert, F.D., Rausch, C., Lehmkuhl, A., Hellmann, I., Smets, M., Leonhardt, H., and Cardoso, M.C. (2017). L1 retrotransposition is activated by Ten-eleven-translocation protein 1 and repressed by methyl-CpG binding proteins. Nucleus 8, 548-562.

Zhang, P., Rausch, C., Hastert, F. D., Boneva, B., Filatova, A., Patil, S. J., Nuber, U. A., Gao, Y., Zhao, X. and Cardoso, M. C. (2017). Methyl-CpG binding domain protein 1 regulates localization and activity of Tet1 in a CXXC3 domain-dependent manner. Nucleic Acids Res 45: 7118-7136.

Rajan, M., Mortusewicz, O., Rothbauer, U., Hastert, F. D., Schmidthals, K., Rapp, A., Leonhardt, H. and Cardoso, M. C. (2015). Generation of an alpaca-derived nanobody recognizing γ-H2AX. FEBS Open Biol 5: 779-788.

 

   
Ksenia Kolobynina

Ksenia Kolobynina joined Cardoso group as a PhD student in July 2017 and is currently focusing on novel regulators of chromatin response to DNA damage. She studied biology at the Kazan Federal University, Russia, to obtain her bachelor degree with a focus on genetics in 2015. Meanwhile she got research experience in the lab of Prof. Dr. Barbara Schmidt in University of Regensburg, Germany, being a part of the project aiming to test HIV mimetic peptides. She did her master thesis at the Institute of Fundamental Medicine and Biology, Kazan, investigating potential role of human CHP3 protein in stem cell differentiation process under supervision of Prof. Albert Rizvanov.

Kolobynina, K. G., Solovyeva, V. V., Levay, K., Rizvanov, A. A. and Slepak, V. Z. (2016). Emerging roles of the single EF-hand Ca2+ sensor tescalcin in the regulation of gene expression, cell growth and differentiation. J Cell Sci 129: 3533-3540.

 

 
Stefania Mamberti

Stefania Mamberti started her Bachelor in Biology at the University of Pavia, Italy, in 2009 and spent six months as an erasmus student at the Ruprecht-Karls-Universität Heidelberg. During her Master Degree in Molecular Biology and Genetics, obtained in Pavia in 2015, she worked on the genetics of Bacillus subtilis and its production of poly-gamma-glutamate.
From the same university she then received a scholarship to identify new larvicidal activities against Aedes albopictus. She started her PhD in the Cardoso group in 2017 to investigate the chromatin structure that underlies processes such as DNA replication and DNA repair in mammalian nuclei.

Mamberti, S., Prati, P., Cremaschi, P., Seppi, C., Morelli, C. F., Galizzi, A., Fabbi, M. and Calvio, C. (2015). γ-PGA
hydrolases of phage origin in Bacillus subtilis and other microbial genomes. PLoS ONE 10.

 

   

Beata Plitta-Michalak (Postdoc) obtained her master’s degree in molecular biology at Adam Mickiewicz University in Poznan, Poland (2006). After she started her PhD studies at the Institute of Bioorganic Chemistry of the Polish academy of Sciences, Poznan, Poland where in January 2013 she obtained a PhD degree cum laude in biochemistry. Her work aimed in searching for new inhibitors of DNA methyltransferases as potential chemotherapeutics in cancer treatment. In February 2013 she joined the Institute of Dendrology of the Polish Academy of Sciences in Kornik, Poland for the post-doc position, where interdisciplinary research concerning plant physiology and seed science is of major interest. She focused on searching for new molecular-based seed quality metrics critical for conservation. In July 2018 she started her post-doc position in Cardoso group to study the impact of solar radiation on DNA. 

Szaflarski, W., Vesper, O., Teraoka, Y., Plitta, B., Wilson, D.N., Nierhaus K.H. (2008). New features of the ribosome and ribosomal inhibitors: non-enzymatic recycling, misreading and back-translocation. J Mol Biol 380: 193-205.

Plitta, B., Adamska, E., Giel-Pietraszuk, M., Fedoruk-Wyszomirska, A., Nasket-Barciszewska, M., Markiewicz, W.T., Barciszewski J. (2012). New cytosine derivatives as inhibitors of DNA methylation. Eur J Med Chem 55: 243-254.

Michalak, M., Barciszewska, M.Z., Barciszewski, J., Plitta, B.P., Chmielarz, P. (2013). Global changes in DNA methylation in seeds and seedlings of Pyrus communis after seed desiccation and storage. Plos ONE 8: e70693.

Michalak, M., Plitta, B.P., Chmielarz, P. (2013). Desiccation sensitivity and successful cryopreservation of oil seeds of European hazelnut (Corylus avellana). Ann of Appl Biol 163: 351-358.

Plitta, B.P., Michalak, M., Naskret-Barciszewska, M.Z., Barciszewski, J., Chmielarz, P. (2014). DNA methylation of Quercus robur L. plumules following cryo-pretreatment and cryopreservation. Plant Cell Tiss Org 117: 31-37.

Suszka, J., Plitta, B.P., Michalak, M., Bujarska-Borkowska, B., Tylkowski, T., Chmielarz, P. (2014). Optimal seed water content and storage temperature for preservation of Populus nigra L. germplasm. Ann For Sci 71: 543-549.

Plitta, B.P., Michalak, M., Bujarska-Borkowska, B., Barciszewska, M.Z., Barciszewski, J., Chmielarz, P. (2014). Effect of desiccation on the dynamics of genom-wide DNA methylation in orthodox seeds of Acer platanoides L. Plant Physiol Bioch 85: 71-77.

Michalak, M., Plitta, B.P., Tylkowski, T., Chmielarz, P., Suszka, J. (2015). Desiccation tolerance and cryopreservation of seeds of black poplar (Populus nigra L.), a disappearing tree species in Europe. Eur J For Res 134: 53-60.

Litkowiec, M., Plitta-Michalak, B.P., Lewandowski, A., Iszkulo, G. (2015). Homogenous genetic structure in populations of Taxus baccata with varied proportions of male and female individuals. Silva Fenn 49: article id 1236.

Michalak, M., Plitta-Michalak, B.P., Bujarska-Borkowska, B., Naskret-Barciszewska, M.Z., Barciszewski, J., Chmielarz, P. (2015). Global 5-methylcytosine alterations in DNA during aging of Quercus robur L. seeds. Ann Bot-London 116: 369-376.

Michalak, M., Plitta-Michalak, B.P., Chmielarz, P. (2015). A new insight in desiccation tolerance and cryopreservation of mazzard cherry (Prunus avium L.) seeds. Open Life Sci 10: 354-364.

Michalak, M., Plitta-Michalak, B.P., Chmielarz, P. (2015) Desiccation tolerance and cryopreservation of wild apple (Malus sylvestris) seeds. Seed Sci Technol 43: 1-12.

Plitta-Michalak, B.P., Naskret-Barciszewska, M.Z., Kotlarski, S., Tomaszewski, D., Tylkowski, T., Barciszewski, J., Chmielarz, P., Michalak, M. (2017). Changes in genomic 5-methylcytosine level mirror the response of orthodox (Acer platanoides L.) and recalcitrant (Acer pseudoplatanus L.) seeds to severe desiccation. Tree Physiol 38: 617-629.

   
Alex

Alexander Rapp (Postdoc) graduated in biochemistry at the Friedrich Schiller University in Jena, Germany, with a diploma thesis on assessing DNA damage after laser micro beams using the Comet-FISH assay.  During this time he got experience on different multi photon laser beams and their use for micromanipulation and damage induction.  After that he did his PhD in Molecular Biology at the Institute of Molecular Biotechnology (IMB) in Jena in the Group of K.O. Greulich (until 2004).  In 2005 he went to the Sir William Dunn School of Pathology, University of Oxford, UK as a Marie Curie Fellow in the lab of P. Cook.  During this time he studied the effects of radiation induced DNA damage in dependency on chromatin structure.  In 2008 he joined the Cardoso group at the TU Darmstadt for his habilitation in the field of DNA repair and chromatin.

Link to publications page

   

Cathia Rausch studied biology at the LMU in Munich and graduated in 2015 in the group of Prof. Dr. Heinrich Leonhardt. In her Master Thesis she investigated the interactome of TET1 and generated as well as characterized several cell lines expressing TET1 (Ten-Eleven-Translocation protein 1) deletion proteins. She joined the Cardoso group in 2016 for her PhD, where she will also focus on TETs and their enzymatic products (especially 5hmC) and investigate their role in DNA replication and chromatin regulation. Furthermore, she will be working on MeCP2 and investigate how post-translational modifications affect localization and protein-protein/protein-DNA interactions.

Natale, F., Scholl, A., Rapp, A., Yu, W., Rausch, C., and Cardoso, M. C. (2018). DNA replication and repair kinetics of Alu, LINE-1 and satellite III genomic repetitive elements. Epigenetics Chromatin 11: 61.

Ludwig, A. K., Zhang, P., Hastert, F. D., Meyer, S., Rausch, C., Herce, H. D., Muller, U., Lehmkuhl, A., Hellmann, I., Trummer, C., Storm, C., Leonhardt, H. and Cardoso, M. C. (2017). Binding of MBD proteins to DNA blocks Tet1 function thereby modulating transcriptional noise. Nucleic Acids Res 45: 2438-2457.

Zhang, P., Ludwig, A. K., Hastert, F. D., Rausch, C., Lehmkuhl, A., Hellmann, I., Smets, M., Leonhardt, H. and Cardoso, M. C. (2017). L1 retrotransposition is activated by Ten-eleven-translocation protein 1 and repressed by methyl-CpG binding proteins. Nucleus 8: 548-562.

Zhang, P., Rausch, C., Hastert, F. D., Boneva, B., Filatova, A., Patil, S. J., Nuber, U. A., Gao, Y., Zhao, X. and Cardoso, M. C. (2017). Methyl-CpG binding domain protein 1 regulates localization and activity of Tet1 in a CXXC3 domain-dependent manner. Nucleic Acids Res 45: 7118-7136.

 

 

Annika Schmidt studied biochemistry at the Ruhr-Universität Bochum and graduated in 2016. During her master thesis at the Medizinisches Proteom-Center Bochum she worked on the establishment and optimization of mass spectrometry methods to identify body fluids in forensic samples. She joined the Cardoso group in 2017 for her PhD to work on MECP2, focusing on post-translational modifications and its influences on MECP2 function.

   
Patrick Weber

Patrick Weber (Paddy) started his studies in biology (B.Sc.) in 2008 at the Technische Universität Darmstadt, Germany and graduated in 2013 with his masterthesis on the ‘DNA replication dynamics in embryonic stem cells‘ in the Cardoso group. He then started his PhD thesis on the same issue with the aim to further identify and characterize differences in the mode of DNA replication and their underlying (epigenetic) regulatory mechanisms. In his spare time he is a passionate dancer and loves to listen to music, hang out with friends and simply enjoy the beautiful things in life :)

Becker, A., Allmann, L., Hofstatter, M., Casa, V., Weber, P., Lehmkuhl, A., Herce, H. D. and Cardoso, M. C. (2013). Direct homo- and hetero-interactions of MeCP2 and MBD2. PLoS One 8: e53730.

 

   

Hui Zhang graduated in Biology at Harbin Institute of Technology (HIT) in China as a Master student in 2017. His Master Thesis involved the functions of Marveld1 (MARVEL domain containing protein 1) during mouse placenta development and underlying molecular mechanisms. In 2017 he joined the Cardoso Lab for his PhD. Here he will focus on the Mecp2 (methyl-CpG binding protein 2) functions during neural differentiation.

Chen, Y., Zhang, H., Han, F., Yue, L., Qiao, C., Zhang, Y., Dou, P., Liu, W. and Li, Y. (2018). The depletion of MARVELD2 leads to murine placenta accreta via integrin β4-dependent trophoblast cell invasion. J. Cell. Physiol 233: 2257-2269.

 

 
 
 
 

Cardoso Lab  •  Technische Universität Darmstadt  •  Germany

Datenschutzerklärung

Back to TUD