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Lab Members

 

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Group Leader

M. Cristina Cardoso graduated in biology at the University of Lisbon, Portugal (1986). After finishing her doctoral thesis on phage molecular genetics (1990), she went on to her post-doctoral training in the group of Dr. B. Nadal-Ginard at the Harvard Medical School (Boston, USA) where she worked on inducing retro-differentiation in terminally differentiated cells. During this time, she started to explore the interplay between the mammalian cell nucleus landscape and (epi)genome metabolism. In 1995, she became a group leader at the Franz Volhard Clinic (Charité-Berlin, Germany) and from 1997 to 2008 at the Max Delbrück Center for Molecular Medicine (Berlin). Since 2008, she has established her group at the Technische Universität Darmstadt.

Link to publications page

   

Secretary

Katrin Schallert is responsible to manage the secretariat of the work group. She is in charge for accounting and secretary matters. Accounting includes in particular the facts and figures of the research projects and in administrative matters. In the secretary, she is responsible among other things, to purchase lab and office material, doing the bills and taking care about personnel matters.

 

 

   
   
Dianca Imblan

Diana Imblan graduated in environment protection at the School of Applied Sciences in Bingen. She is working as a technical assistant in the Cardoso lab and takes care of several databases (Library of papers, Inventory lists, Peptides) and is supporting Manuela in protein purification.

   

Anne Lehmkuhl takes the best care of our cell cultures and is therefore absolutely not replaceable. She is all ears for special wishes and keeps the lab running with all the time freshly prepared solutions and sterilized lab materials.

Ludwig, A. K.#, Zhang, P.#, Hastert, F. D., Meyer, S., Rausch, C., Herce, H. D., Muller, U., Lehmkuhl, A., Hellmann, I., Trummer, C., Storm, C., Leonhardt, H. and Cardoso, M. C. (2017). Binding of MBD proteins to DNA blocks Tet1 function thereby modulating transcriptional noise. Nucleic Acids Res 45: 2438-2457.

Zhang, P.#, Ludwig, A. K.#, Hastert, F. D., Rausch, C., Lehmkuhl, A., Hellmann, I., Smets, M., Leonhardt, H. and Cardoso, M. C. (2017). L1 retrotransposition is activated by Ten-eleven-translocation protein 1 and repressed by methyl-CpG binding proteins. Nucleus 8: 548-562.

Becker, A., Allmann, L., Hofstatter, M., Casa, V., Weber, P., Lehmkuhl, A., Herce, H. D. and Cardoso, M. C. (2013). Direct homo- and hetero-interactions of MeCP2 and MBD2. PLoS One 8: e53730.

Bertulat, B., De Bonis, M. L., Della Ragione, F., Lehmkuhl, A., Milden, M., Storm, C., Jost, K. L., Scala, S., Hendrich, B., D'esposito, M. and Cardoso, M. C. (2012). MeCP2 dependent heterochromatin reorganization during neural differentiation of a novel Mecp2-deficient embryonic stem cell reporter line. PLoS One 7: e47848.

 

   
Manu

Manuela Milden studied Biotechnology at the University of Applied Sciences Darmstadt, Germany. Her diploma thesis was finished in 2008 in the department of molecular biology. Now she takes care of the biochemistry lab, the antibodies and our Äkta system.

Bertulat, B., De Bonis, M. L., Della Ragione, F., Lehmkuhl, A., Milden, M., Storm, C., Jost, K. L., Scala, S., Hendrich, B., D'esposito, M. and Cardoso, M. C. (2012). MeCP2 dependent heterochromatin reorganization during neural differentiation of a novel Mecp2-deficient embryonic stem cell reporter line. PLoS One 7: e47848.

Jost, K. L., Rottach, A., Milden, M., Bertulat, B., Becker, A., Wolf, P., Sandoval, J., Petazzi, P., Huertas, D., Esteller, M., Kremmer, E., Leonhardt, H. and Cardoso, M. C. (2011). Generation and Characterization of Rat and Mouse Monoclonal Antibodies Specific for MeCP2 and Their Use in X-Inactivation Studies. PLoS One 6: e26499.

 

 
   
Bianca

Bianca Bertulat (Postdoc) graduated with a focus on molecular and developmental cell biology at the Technische Universität Darmstadt (2002). For her PhD (2008) she switched topics from mesoderm evolution to neural stem cell specification and moved with the Holstein lab to the University of Heidelberg. There, she finished her practical work, focusing on the identification and characterisation of basic Helix-Loop-Helix transcription factors (supervised by Thomas Holstein). Bianca joined the Cardoso lab 2009, eager to learn all about MeCP2, chromocenters, and nuclear architecture.

plays guitar with the BiophoniX

Erbeldinger, N., Rapp, F., Ktitareva, S., Wendel, P., Bothe, A. S., Dettmering, T., Durante, M., Friedrich, T., Bertulat, B., Meyer, S., Cardoso, M. C., Hehlgans, S., Rodel, F. and Fournier, C. (2017). Measuring Leukocyte Adhesion to (Primary) Endothelial Cells after Photon and Charged Particle Exposure with a Dedicated Laminar Flow Chamber. Front Immunol 8: 627.

Becker, A., Zhang, P., Allmann, L., Meilinger, D., Bertulat, B., Eck, D., Hofstaetter, M., Bartolomei, G., Hottiger, M. O., Schreiber, V., Leonhardt, H. and Cardoso, M. C. (2016). Poly(ADP-ribosyl)ation of Methyl CpG Binding Domain Protein 2 Regulates Chromatin Structure. J Biol Chem 291: 4873-4881.

Jost, K. L., Bertulat, B., Rapp, A., Brero, A., Hardt, T., Domaing, P., Gosele, C., Schulz, H., Hubner, N. and Cardoso, M. C. (2015). Gene repositioning within the cell nucleus is not random and is determined by its genomic neighborhood. Epigenetics Chromatin 8: 36.

Bertulat, B., De Bonis, M. L., Della Ragione, F., Lehmkuhl, A., Milden, M., Storm, C., Jost, K. L., Scala, S., Hendrich, B., D'esposito, M. and Cardoso, M. C. (2012). MeCP2 dependent heterochromatin reorganization during neural differentiation of a novel Mecp2-deficient embryonic stem cell reporter line. PLoS One 7: e47848.

Jost, K. L., Bertulat, B. and Cardoso, M. C. (2012). Heterochromatin and gene positioning: inside, outside, any side? Chromosoma 121: 555-563.

Jost, K. L., Rottach, A., Milden, M., Bertulat, B., Becker, A., Wolf, P., Sandoval, J., Petazzi, P., Huertas, D., Esteller, M., Kremmer, E., Leonhardt, H. and Cardoso, M. C. (2011). Generation and Characterization of Rat and Mouse Monoclonal Antibodies Specific for MeCP2 and Their Use in X-Inactivation Studies. PLoS One 6: e26499.

Jost, K. L., Haase, S., Smeets, D., Schrode, N., Schmiedel, J. M., Bertulat, B., Herzel, H., Cremer, M. and Cardoso, M. C. (2011). 3D-Image analysis platform monitoring relocation of pluripotency genes during reprogramming. Nucleic Acids Res 39: e113.

Chapman JA et al. (2010). The dynamic genome of Hydra. Nature 464: 592-6.

 

   

Hadassa Campos-Heiser graduated in Biomedicine at the Centro Universitário de Maringá (UniCesumar) (2012) and hold a Master’s degree in Medical Science from the Medical School of the University of Sao Paulo (2015). After the Master she worked as Research Assistant in Population Genetics and Bioinformatics at the Laboratory of Genetics and Molecular Cardiology (LGCM-InCor). In 2017 she joined the Cardoso Lab where she is working on the identification and epigenetic characterization of the Origins of DNA Replication. Besides her scientific work she also enjoys photography, dancing and reading topics such as history, economics, physics and stars. She is a nature lover.

 

   

Emilia Rita De Caro is currently contributing to an interdisciplinary project of the departments of biology and material sciences and engineering. Aim of the collaboration is to establish a surface biology-testing unit (SuBiTU) that allows standardized in vitro testing of bone-implant surfaces. As a tool for implant characteriszation, SuBiTU will help to reduce animal testing and enable us to monitor early implant-surface-cell interactions. To this end we developed an implant suitable live cell chamber, established a stable cell lines, and currently optimizing 3D cell culture techniques for live cell microscopy.

 
Florian Hastert

Florian Hastert got both, his bachelor's and master's degree, from the TU Darmstadt. His bachelor's thesis was completed at the "GSI Helmholtz Centre for Heavy Ion Research" on the recruitment of proteins involved in chromatin remodeling following radiation induced DNA damage. In his master's thesis in the Cardoso lab he investigated the interplay of histone and DNA modifications. This is now also keeping him busy during his PhD.

plays drums with the BiophoniX

Zhang, P., Hastert, F. D., Ludwig, A. K., Breitwieser, K., Hofstätter, M. and Cardoso, M. C. (2017). DNA base flipping analytical pipeline, Biology Methods and Protocols 2: 1-12

Ludwig, A. K., Zhang, P., Hastert, F. D., Meyer, S., Rausch, C., Herce, H. D., Muller, U., Lehmkuhl, A., Hellmann, I., Trummer, C., Storm, C., Leonhardt, H. and Cardoso, M. C. (2017). Binding of MBD proteins to DNA blocks Tet1 function thereby modulating transcriptional noise. Nucleic Acids Res 45: 2438-2457.

Zhang, P., Ludwig, A.K., Hastert, F.D., Rausch, C., Lehmkuhl, A., Hellmann, I., Smets, M., Leonhardt, H., and Cardoso, M.C. (2017). L1 retrotransposition is activated by Ten-eleven-translocation protein 1 and repressed by methyl-CpG binding proteins. Nucleus 8, 548-562.

Zhang, P., Rausch, C., Hastert, F. D., Boneva, B., Filatova, A., Patil, S. J., Nuber, U. A., Gao, Y., Zhao, X. and Cardoso, M. C. (2017). Methyl-CpG binding domain protein 1 regulates localization and activity of Tet1 in a CXXC3 domain-dependent manner. Nucleic Acids Res 45: 7118-7136.

Rajan, M., Mortusewicz, O., Rothbauer, U., Hastert, F. D., Schmidthals, K., Rapp, A., Leonhardt, H. and Cardoso, M. C. (2015). Generation of an alpaca-derived nanobody recognizing γ-H2AX. FEBS Open Biol 5: 779-788.

 

   
   
Kathrin Heinz

Kathrin Heinz graduated in Biology at the Technische Universität Darmstadt (2013). She joined the Cardoso lab already in 2011 to write her Bachelor Thesis on physiological effects of Cell-penetrating peptides derived from p53 and p21. In 2013 she starts her Master Thesis on heterochromatic (com)position on DNA replication timing, which is the fundament of her current Ph.D. studies. Beyond her scientific work, she is very interested in teaching and therefore was involved in all teaching modules of the lab, as well as she is giving lectures at the ‘Pädagogische Akademie Elisabethenstift’ in Darmstadt.

Heinz, K. S., Casas-Delucchi, C. S., Török, T., Cmarko, D., Rapp, A., Raska, I. and Cardoso, M. C. (2018). Peripheral re-localization of constitutive heterochromatin advances its replication timing and impairs maintenance of silencing marks. Nucleic Acids Res. doi: 10.1093/nar/gky368.

 

   
Ksenia Kolobynina

Ksenia Kolobynina joined Cardoso group as a PhD student in July 2017 and is currently focusing on novel regulators of chromatin response to DNA damage. She studied biology at the Kazan Federal University, Russia, to obtain her bachelor degree with a focus on genetics in 2015. Meanwhile she got research experience in the lab of Prof. Dr. Barbara Schmidt in University of Regensburg, Germany, being a part of the project aiming to test HIV mimetic peptides. She did her master thesis at the Institute of Fundamental Medicine and Biology, Kazan, investigating potential role of human CHP3 protein in stem cell differentiation process under supervision of Prof. Albert Rizvanov.

Kolobynina, K. G., Solovyeva, V. V., Levay, K., Rizvanov, A. A. and Slepak, V. Z. (2016). Emerging roles of the single EF-hand Ca2+ sensor tescalcin in the regulation of gene expression, cell growth and differentiation. J Cell Sci. 129: 3533-3540.

 

 
Stefania Mamberti

Stefania Mamberti started her Bachelor in Biology at the University of Pavia, Italy, in 2009 and spent six months as an erasmus student at the Ruprecht-Karls-Universität Heidelberg. During her Master Degree in Molecular Biology and Genetics, obtained in Pavia in 2015, she worked on the genetics of Bacillus subtilis and its production of poly-gamma-glutamate.
From the same university she then received a scholarship to identify new larvicidal activities against Aedes albopictus. She started her PhD in the Cardoso group in 2017 to investigate the chromatin structure that underlies processes such as DNA replication and DNA repair in mammalian nuclei.

Mamberti, S., Prati, P., Cremaschi, P., Seppi, C., Morelli, C. F., Galizzi, A., Fabbi, M. and Calvio, C. (2015). γ-PGA
hydrolases of phage origin in Bacillus subtilis and other microbial genomes. PLoS ONE 10.

 

   
Alex

Alexander Rapp (Postdoc) graduated in biochemistry at the Friedrich Schiller University in Jena, Germany, with a diploma thesis on assessing DNA damage after laser micro beams using the Comet-FISH assay.  During this time he got experience on different multi photon laser beams and their use for micromanipulation and damage induction.  After that he did his PhD in Molecular Biology at the Institute of Molecular Biotechnology (IMB) in Jena in the Group of K.O. Greulich (until 2004).  In 2005 he went to the Sir William Dunn School of Pathology, University of Oxford, UK as a Marie Curie Fellow in the lab of P. Cook.  During this time he studied the effects of radiation induced DNA damage in dependency on chromatin structure.  In 2008 he joined the Cardoso group at the TU Darmstadt for his habilitation in the field of DNA repair and chromatin.

Link to publications page

   

Cathia Rausch studied biology at the LMU in Munich and graduated in 2015 in the group of Prof. Dr. Heinrich Leonhardt. In her Master Thesis she investigated the interactome of TET1 and generated as well as characterized several cell lines expressing TET1 (Ten-Eleven-Translocation protein 1) deletion proteins. She joined the Cardoso group in 2016 for her PhD, where she will also focus on TETs and their enzymatic products (especially 5hmC) and investigate their role in DNA replication and chromatin regulation. Furthermore, she will be working on MeCP2 and investigate how post-translational modifications affect localization and protein-protein/protein-DNA interactions.

Ludwig, A. K., Zhang, P., Hastert, F. D., Meyer, S., Rausch, C., Herce, H. D., Muller, U., Lehmkuhl, A., Hellmann, I., Trummer, C., Storm, C., Leonhardt, H. and Cardoso, M. C. (2017). Binding of MBD proteins to DNA blocks Tet1 function thereby modulating transcriptional noise. Nucleic Acids Res 45: 2438-2457.

Zhang, P., Ludwig, A. K., Hastert, F. D., Rausch, C., Lehmkuhl, A., Hellmann, I., Smets, M., Leonhardt, H. and Cardoso, M. C. (2017). L1 retrotransposition is activated by Ten-eleven-translocation protein 1 and repressed by methyl-CpG binding proteins. Nucleus 8: 548-562.

Zhang, P., Rausch, C., Hastert, F. D., Boneva, B., Filatova, A., Patil, S. J., Nuber, U. A., Gao, Y., Zhao, X. and Cardoso, M. C. (2017). Methyl-CpG binding domain protein 1 regulates localization and activity of Tet1 in a CXXC3 domain-dependent manner. Nucleic Acids Res 45: 7118-7136.

 

 

Annika Schmidt studied biochemistry at the Ruhr-Universität Bochum and graduated in 2016. During her master thesis at the Medizinisches Proteom-Center Bochum she worked on the establishment and optimization of mass spectrometry methods to identify body fluids in forensic samples. She joined the Cardoso group in 2017 for her PhD to work on MECP2, focusing on post-translational modifications and its influences on MECP2 function.

   
Nicola

Nicola Stricker studied biology at the TU Darmstadt and graduated in 2014. In her Master Thesis she optimized in vitro culture and expansion of natural killer cells for treatment of acute myeloid leukemia at the Institute for Transfusion Medicine and immunohaematology in Frankfurt/ Main. She joined the Cardoso-Lab in 2014 for her PhD, which will be focused on the combined effects of UV, infrared and visible light on DNA damage induction and repair.

   
Patrick Weber

Patrick Weber (Paddy) started his studies in biology (B.Sc.) in 2008 at the Technische Universität Darmstadt, Germany and graduated in 2013 with his masterthesis on the ‘DNA replication dynamics in embryonic stem cells‘ in the Cardoso group. He then started his PhD thesis on the same issue with the aim to further identify and characterize differences in the mode of DNA replication and their underlying (epigenetic) regulatory mechanisms. In his spare time he is a passionate dancer and loves to listen to music, hang out with friends and simply enjoy the beautiful things in life :)

Becker, A., Allmann, L., Hofstatter, M., Casa, V., Weber, P., Lehmkuhl, A., Herce, H. D. and Cardoso, M. C. (2013). Direct homo- and hetero-interactions of MeCP2 and MBD2. PLoS One 8: e53730.

 

   

Hui Zhang graduated in Biology at Harbin Institute of Technology (HIT) in China as a Master student in 2017. His Master Thesis involved the functions of Marveld1 (MARVEL domain containing protein 1) during mouse placenta development and underlying molecular mechanisms. In 2017 he joined the Cardoso Lab for his PhD. Here he will focus on the Mecp2 (methyl-CpG binding protein 2) functions during neural differentiation.

Chen, Y., Zhang, H., Han, F., Yue, L., Qiao, C., Zhang, Y., Dou, P., Liu, W. and Li, Y. (2018). The depletion of MARVELD2 leads to murine placenta accreta via integrin β4-dependent trophoblast cell invasion. J. Cell. Physiol. 233: 2257-2269.

 

 
 
 
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